Journal: The Journal of Biological Chemistry

Article Title: Rate and Regulation of Copper Transport by Human Copper Transporter 1 (hCTR1) *

doi: 10.1074/jbc.M112.442426

Figure Lengend Snippet: Confocal imaging of cells treated with copper. Cells were treated with normal media or media containing 25 μm copper for 60 min prior to fixation and staining with mouse anti-FLAG (green, detects hCTR1) and rabbit anti-β-catenin (red, β-catenin is primarily found in membranes). In each panel, cells were overexpressing the following: A–C, WT hCTR1, no added copper in the media; D–F, WT hCTR1, 25 μm added copper in media; G–I, hCTR1 AAA mutant, no added copper in the media; J–L, hCTR1 AAA mutant, 25 μm added copper in media.

Article Snippet: The following primary antibodies were used: rabbit anti-hCTR1 antibody against hCTR1 C-terminal tail ( 10 ), mouse anti-FLAG (GenScript, Piscataway, NJ), rabbit anti-FLAG (Sigma-Aldrich), mouse anti-Na,K-ATPase β1 (Affinity Bioreagents, Golden, CO), mouse anti-β-catenin (BD Biosciences), and mouse anti-α1 subunit of Na,K-ATPase (Affinity Bioreagents).

Techniques: Imaging, Staining, Mutagenesis